实验室：流式平台

职位：平台主管

西湖大学（云栖校区）3号楼512室

西湖大学（云谷校区）学术环E10-223

15863398535/0571-85273905

dingxilai@westlake.edu.cn

教育背景

1999.9-2003.7山东师范大学，应用化学，本科

2003.9-2010.1中国科学院生物物理研究所，生物化学与分子生物学，理学博士

2011.4-2012.4 The Roswell Park Cancer Institute, Buffalo, NY，Postdoc

2012.5-2015.9 Methodists Hospital Research Institute, Houston, TX，Postdoc

工作经历

2016.1-2018.9临沂大学 生化分析研究所 讲师

2018.7.2-至今西湖大学 生物医学实验中心 流式平台主管

工作职责

制订平台各类分析或分选仪器的日常使用和周期性维护SOP；制订流式样本制备、染色、上机、数据分析的用户培训方案；开发基于流式细胞检测原理的新技术和新方法；为用户个性化实验设计和数据分析等提供咨询和建议；关注流式技术创新和仪器发展趋势，为平台建设和发展提供专业建议。

发表文章

High-multiplex single-cell imaging analysis reveals tumor immune contexture associated with clinical outcomes after CAR T cell therapy.

Jin J, Lin L, Meng J, Jiang L, Zhang M, Fang Y, Liu W, Xin X, Long X, Kuang D, Ding X, Zheng M, Zhang Y, Xiao Y, Chen L.

(Molecular Therapy. 2024 May 1;32(5):1252-1265. doi: 10.1016/j.ymthe.2024.03.023.)

Rapid and label-free detection of aflatoxin B1 using a rationally truncated aptamer andviacircular dichroism measurement.

Wang C, Zhu K, Shi P,Ding X, Zhang S.

(Chem Commun. 2022 Apr 12;58(30):4779-4782. doi: 10.1039/d2cc00078d.)

Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma.

Zhu M,Ding X, Zhao R, Liu X, Shen H, Cai C, Ferrari M, Wang HY, Wang RF.

J Control Release. 2018 Feb 28;272:72-82. doi: 10.1016/j.jconrel.2018.01.004. Epub 2018 Jan 8.

Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation.

Li Q, Zou J, Wang M,Ding X, Chepelev I, Zhou X, Zhao W, Wei G, Cui J, Zhao K, Wang HY, Wang RF.

Nat Commun. 2014 Dec 22;5:5780. doi: 10.1038/ncomms6780.

A flagellin-derived toll-like receptor 5 agonist stimulates cytotoxic lymphocyte-mediated tumor immunity.

Leigh ND, Bian G,Ding X, Liu H, Aygun-Sunar S, Burdelya LG, Gudkov AV, Cao X.

PLoS One. 2014 Jan 14;9(1):e85587. doi: 10.1371/journal.pone.0085587. eCollection 2014.

Gzm B deficiency enhance Graftversus-Tumor Immunity.

Bian G*,Ding X*, Burdelya L, McCarthy P, Gudkov A, Cao X.

(Journal of Immunology, 2013, 190:1341-1350)

A TLR5 agonist enhances CD8(+) T cell-mediated graft-versus-tumor effect without exacerbating graft-versus-host disease.

Ding X, Bian G, Leigh ND, Qiu J, McCarthy PL, Liu H, Aygun-Sunar S, Burdelya LG, Gudkov AV, Cao X.

J Immunol. 2012 Nov 15;189(10):4719-27. doi: 10.4049/jimmunol.1201206. Epub 2012 Oct 8.

Tumor Necrosis Factor Receptor 1 Mediates Dendritic Cell Maturation and CD8 T Cell Response Through Two Distinct Mechanisms.

Ding X,Yang W, Shi X, Du P, Su L, Qin Z, Chen J, Deng H. 2010.

(Journal of Immunology. 2011 Aug 1; 187 (3):1184-91)

Interferon-gamma negatively regulates Th17-mediated immunopathology during mouse hepatitis virus infection.

Yang W,Ding X, Deng J, Lu Y, Matsuda Z, Thiel A, Chen J, Deng H, Qin Z.

J Mol Med (Berl). 2011 Apr;89(4):399-409. doi: 10.1007/s00109-010-0711-5. Epub 2010 Dec 30.

Rapid estimation of binding activity of influenza virus hemagglutinin to human and avian receptors.

Cao Y, Koh X, Dong L, Du X, Wu A,Ding X, Deng H, Shu Y, Chen J, Jiang T.

PLoS One. 2011 Apr 13;6(4):e18664. doi: 10.1371/journal.pone.0018664.

Circulating and liver resident CD4+CD25+ regulatory T cells actively influence the antiviral immune response and disease progression in patients with hepatitis B.

Xu D, Fu J, Jin L, Zhang H, Zhou C, Zou Z, Zhao JM, Zhang B, Shi M,Ding X, Tang Z, Fu YX, Wang FS.

J Immunol. 2006 Jul 1;177(1):739-47.

Cross-Regulation of Two Type I Interferon Signaling Pathways in Plasmacytoid Dendritic Cells Controls Anti-malaria Immunity and Host Mortality.

Yu X, Cai B, Wang M, Tan P,Ding X, Wu J, Li J, Li Q, Liu P, Xing C, Wang HY, Su XZ, Wang RF.

Immunity. 2016 Nov 15;45(5):1093-1107. doi: 10.1016/j.immuni.2016.10.001. Epub 2016 Oct 25.

The significance of comprehensive staging surgery in malignant ovarian germ cell tumors.

Liu Q,Ding X, Yang J, Cao D, Shen K, Lang J, Zhang G, Xin X, Xie X, Wu Y.

Gynecol Oncol. 2013 Dec;131(3):551-4. doi: 10.1016/j.ygyno.2013.08.016. Epub 2013 Sep 2.

Granzyme B-mediated damage of CD8+ T cells impairs graft-versus-tumor effect.

Bian G,Ding X, Leigh ND, Tang Y, Capitano ML, Qiu J, McCarthy PL, Liu H, Cao X.

J Immunol. 2013 Feb 1;190(3):1341-50. doi: 10.4049/jimmunol.1201554. Epub 2012 Dec 21.

A TLR5 agonist enhances CD8(+) T cell-mediated graft-versus-tumor effect without exacerbating graft-versus-host disease.

Ding X, Bian G, Leigh ND, Qiu J, McCarthy PL, Liu H, Aygun-Sunar S, Burdelya LG, Gudkov AV, Cao X.

J Immunol. 2012 Nov 15;189(10):4719-27. doi: 10.4049/jimmunol.1201206. Epub 2012 Oct 8.

MGB-based one-step multiplex real-time PCR method for rapid detection of HPV.

Huang J, Gao C,Ding X, Qu S, Liu L, Wu W, Zhang L, Zhao J, Gao S.

Cancer Biomark. 2012-2013;12(3):107-13. doi: 10.3233/CBM-130298.

TNF receptor 1 mediates dendritic cell maturation and CD8 T cell response through two distinct mechanisms.

Ding X, Yang W, Shi X, Du P, Su L, Qin Z, Chen J, Deng H.

J Immunol. 2011 Aug 1;187(3):1184-91. doi: 10.4049/jimmunol.1002902. Epub 2011 Jun 27.

Rapid estimation of binding activity of influenza virus hemagglutinin to human and avian receptors.

Cao Y, Koh X, Dong L, Du X, Wu A,Ding X, Deng H, Shu Y, Chen J, Jiang T.

PLoS One. 2011 Apr 13;6(4):e18664. doi: 10.1371/journal.pone.0018664.

Interferon-gamma negatively regulates Th17-mediated immunopathology during mouse hepatitis virus infection.

Yang W,Ding X, Deng J, Lu Y, Matsuda Z, Thiel A, Chen J, Deng H, Qin Z.

J Mol Med (Berl). 2011 Apr;89(4):399-409. doi: 10.1007/s00109-010-0711-5. Epub 2010 Dec 30.